[Homeroast] Six words describing why you love coffee

Brian Kamnetz bkamnetz at gmail.com
Fri Jul 16 10:12:12 CDT 2010


Good one, Ray!

Brian


On Fri, Jul 16, 2010 at 12:54 AM,  <raymanowen at gmail.com> wrote:
> "bovine lactation will not defile you..."
>
> Bovine Lactate is half bad.
> According to *Science Direct*, Volume 91, Issue
> 2<http://www.sciencedirect.com/science?_ob=PublicationURL&_tockey=%23TOC%234941%231990%23999089997%23383409%23FLP%23&_cdi=4941&_pubType=J&view=c&_auth=y&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=28a52319cb02fa93469ec1252dd9ff58>,
> of 16 July 1990, Pages 281-285, here are the components:
>
> "Primary structure of bovine lactate dehydrogenase-A isozyme and its
> synthesis in *Escherichia coli-*
> [image: Purchase the full-text
> article]<http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T39-47T2H0W-P-1&_cdi=4941&_user=10&_pii=037811199090101V&_orig=search&_coverDate=07%2F16%2F1990&_sk=999089997&view=c&wchp=dGLzVzb-zSkzS&md5=743bee3b0a48262cd124e6ca4baeea2c&ie=/sdarticle.pdf>
> *
>
> Naotaka Ishiguroa<http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-47T2H0W-P&_user=10&_coverDate=07%2F16%2F1990&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1402078322&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=c5347ca4809bb0e64d0ae5dea3325e26#aff1>
> , [image: Corresponding Author Contact
> Information]<http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-47T2H0W-P&_user=10&_coverDate=07%2F16%2F1990&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1402078322&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=c5347ca4809bb0e64d0ae5dea3325e26#m4.cor*>,
> Satoshi Osameb<http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-47T2H0W-P&_user=10&_coverDate=07%2F16%2F1990&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1402078322&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=c5347ca4809bb0e64d0ae5dea3325e26#aff2>,
> Ryo Kagiyab<http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-47T2H0W-P&_user=10&_coverDate=07%2F16%2F1990&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1402078322&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=c5347ca4809bb0e64d0ae5dea3325e26#aff2>,
> Shigeru Ichijob<http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-47T2H0W-P&_user=10&_coverDate=07%2F16%2F1990&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1402078322&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=c5347ca4809bb0e64d0ae5dea3325e26#aff2>
> and
> Morikazu Shinagawaa<http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-47T2H0W-P&_user=10&_coverDate=07%2F16%2F1990&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1402078322&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=c5347ca4809bb0e64d0ae5dea3325e26#aff1>
> *
>
> a Department of Veterinary Public Health, Obihiro University of Agriculture
> and Veterinary Medicine, Obihiro 080, Japan
>
> b Department of Veterinary Internal Medicine, Obihiro University of
> Agriculture and Veterinary Medicine, Obihiro 080, Japan
> Received 27 January 1990;
> revised 22 March 1990;
> accepted 9 April 1990;
> Received by A. Nakazawa
> Available online 28 January 2003.
>
> Abstract
> Eleven cDNA clones encoding lactate dehydrogenase(LDH)-A isozyme were
> isolated from a bovine lymphocyte cDNA library, and the nucleotide sequences
> of three of the clones (pLDH5, pLDH9 and pLDH12) were determined.
>
> With the exception of variation in the 5′ portion, two cDNA clones (pLDH9
> and pLDH12) appeared to contain the full-length cDNA of 1786 bp, consisting
> of the protein-coding sequence (996 bp), the 5′- and the 3′-untranslated
> regions and the poly(dA) tail.
>
> The predicted amino acid (aa) sequence of bovine LDH-A (332 aa) showed 96.7%
> homology with that of pig LDH-A.
>
> The protein-coding cDNA region (1650 bp) was inserted into an *Escherichia
> coli* expression vector ptac11 and expressed.
>
> The protein synthesized *E. coli* showed enzyme activity of LDH and was
> identified by cellogel electrophoresis as LDH-5 isozyme whose subunit M
> chain is the product of the *LDH-A* gene."
>
> [What's in your cup? Could you even diagram one of those sentences?]
>
> Cheers, Mabuhay -RayO, aka Opa!
>
> Ich fragte mich- wie schmekt diesen Scheißegeschmack?
>
> . . .
>> monkey [with] my cup [at your own peril]
>>
>>
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