[Homeroast] Six words describing why you love coffee

raymanowen at gmail.com raymanowen at gmail.com
Thu Jul 15 23:54:47 CDT 2010


"bovine lactation will not defile you..."

Bovine Lactate is half bad.
According to *Science Direct*, Volume 91, Issue
2<http://www.sciencedirect.com/science?_ob=PublicationURL&_tockey=%23TOC%234941%231990%23999089997%23383409%23FLP%23&_cdi=4941&_pubType=J&view=c&_auth=y&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=28a52319cb02fa93469ec1252dd9ff58>,
of 16 July 1990, Pages 281-285, here are the components:

"Primary structure of bovine lactate dehydrogenase-A isozyme and its
synthesis in *Escherichia coli-*
[image: Purchase the full-text
article]<http://www.sciencedirect.com/science?_ob=MImg&_imagekey=B6T39-47T2H0W-P-1&_cdi=4941&_user=10&_pii=037811199090101V&_orig=search&_coverDate=07%2F16%2F1990&_sk=999089997&view=c&wchp=dGLzVzb-zSkzS&md5=743bee3b0a48262cd124e6ca4baeea2c&ie=/sdarticle.pdf>
*

Naotaka Ishiguroa<http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-47T2H0W-P&_user=10&_coverDate=07%2F16%2F1990&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1402078322&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=c5347ca4809bb0e64d0ae5dea3325e26#aff1>
, [image: Corresponding Author Contact
Information]<http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-47T2H0W-P&_user=10&_coverDate=07%2F16%2F1990&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1402078322&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=c5347ca4809bb0e64d0ae5dea3325e26#m4.cor*>,
Satoshi Osameb<http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-47T2H0W-P&_user=10&_coverDate=07%2F16%2F1990&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1402078322&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=c5347ca4809bb0e64d0ae5dea3325e26#aff2>,
Ryo Kagiyab<http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-47T2H0W-P&_user=10&_coverDate=07%2F16%2F1990&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1402078322&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=c5347ca4809bb0e64d0ae5dea3325e26#aff2>,
Shigeru Ichijob<http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-47T2H0W-P&_user=10&_coverDate=07%2F16%2F1990&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1402078322&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=c5347ca4809bb0e64d0ae5dea3325e26#aff2>
and
Morikazu Shinagawaa<http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T39-47T2H0W-P&_user=10&_coverDate=07%2F16%2F1990&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1402078322&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=c5347ca4809bb0e64d0ae5dea3325e26#aff1>
*

a Department of Veterinary Public Health, Obihiro University of Agriculture
and Veterinary Medicine, Obihiro 080, Japan

b Department of Veterinary Internal Medicine, Obihiro University of
Agriculture and Veterinary Medicine, Obihiro 080, Japan
Received 27 January 1990;
revised 22 March 1990;
accepted 9 April 1990;
Received by A. Nakazawa
Available online 28 January 2003.

Abstract
Eleven cDNA clones encoding lactate dehydrogenase(LDH)-A isozyme were
isolated from a bovine lymphocyte cDNA library, and the nucleotide sequences
of three of the clones (pLDH5, pLDH9 and pLDH12) were determined.

With the exception of variation in the 5′ portion, two cDNA clones (pLDH9
and pLDH12) appeared to contain the full-length cDNA of 1786 bp, consisting
of the protein-coding sequence (996 bp), the 5′- and the 3′-untranslated
regions and the poly(dA) tail.

The predicted amino acid (aa) sequence of bovine LDH-A (332 aa) showed 96.7%
homology with that of pig LDH-A.

The protein-coding cDNA region (1650 bp) was inserted into an *Escherichia
coli* expression vector ptac11 and expressed.

The protein synthesized *E. coli* showed enzyme activity of LDH and was
identified by cellogel electrophoresis as LDH-5 isozyme whose subunit M
chain is the product of the *LDH-A* gene."

[What's in your cup? Could you even diagram one of those sentences?]

Cheers, Mabuhay -RayO, aka Opa!

Ich fragte mich- wie schmekt diesen Scheißegeschmack?

. . .
> monkey [with] my cup [at your own peril]
>
>


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